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KMID : 0380019950100020196
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Korean Journal of Biotechnology and Bioengineering
1995 Volume.10 No. 2 p.196 ~ p.203
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Effect of Nutrients and Applications for the Overproduction of Polyoxins by Streptomyces Speices
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Abstract
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The effects of major nutrients and environmental factors on polyoxins biosysnthesis were examined for the overproduction of antifungal agent polyoxins using Streptomyces sp. 809-11. The cell mass at the exponential growth phase was increased when soluble starch was used, while the polyoxins biosynthesis was increased with the formation of filamentous mycelium when glucose was used as a carbon source. It was, therefore, recommendable to use both soluble starch and glucose simultaneously as carbon sources for the cell growth and polyoxins production. The high concentration of ammonium sulfate (151.4 mM) resulted in the improvement of polyoxins production. The optimal concentration of K2HPO,
,for polyoxins production was found to be 0.5mM. By applying fed-batch culture, polyoxins production was improved to about 2-folds compared to the result from the batch culture.
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